Container for culturing, micro manipulation and identification of small specimens

ABSTRACT

A disposable polymer container for manipulation of small specimens adapted to optimize heat transfer between an external heating element and specimens herein contained. In particular, this invention relates to the field of containers for Assisted Reproductive Technology hereunder In-vitro fertilization (IVF).

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. patent application Ser. No.14/939,941, filed Nov. 12, 2019, which is a continuation of U.S. patentapplication Ser. No. 13/878,487, filed Jun. 20, 2013, which claimspriority to PCT Int'l App. No. PCT/DK2011/050401, filed Oct. 21, 2011,which claims the benefit of priority to Danish Pat. App. No. PA 201070447, filed Oct. 22, 2010, and U.S. Provisional App. No. 61/405,956,filed on Oct. 22, 2010. The disclosures of the above-referencedapplications are hereby expressly incorporated by reference in theirentireties.

BACKGROUND OF THE INVENTION Field of the Invention

This invention relates to the field of containers for culturing andmanipulating small specimens.

In particular, this invention relates to the field of containers forAssisted Reproductive Technology hereunder In-vitro fertilization (IVF).

Description of the Related Art

Disposable sterile polymer containers such dishes and well plates areuseful when manipulating smaller specimens in liquid media, e.g duringartificial reproductive technologies such as In-Vitro Fertilisation(IVF) by the micro manipulation and culturing of spermatozoa, oocytesand embryos.

Current containers used for micro manipulation within IVF are mostlygeneric, off-the-shelf, dishes developed for standard tissue culture.The micro manipulation of immature oocytes, mature oocytes, gametes,zygotes, embryos, cleavage stage embryos, blastocyst stage embryos,precursor cells, and such, referred to herein as “specimens”, are donein standard 35 mm, 50 mm or 60 mm Petri Dish or 4 Well Dish, containerswith few unique features, each dish or well simply has flat transparentbottoms and substantially vertical side walls.

Flat and transparent bottom is important for most applications as itfacilitates the microscopy of the biological material within the dish orwell. One additional feature of these generic dishes and well plates isthe elevation of the flat bottom surface from the working table by arim. This rim being at the circumference below the bottom surface andhas generally the advantage of reinforcing the strength of the dish orwell plate to reduce internal stresses and avoid distortion of the flatbottom during solidification of the polymer article during production.

Further the rim elevates the bottom to avoid scratching of the bottomsurface during stacking of the dish or well plate for packaging,transportation and during incubation. Scratching reduces visibility ofthe specimens during use and during microscopy.

Within the field of IVF, the human oocytes and embryos must be held asclosely as possible at a stable 37 degrees Celsius. The ability tocontrol the temperature of the bottom of the dish and so also thetemperature of the embryos and oocytes has proven to be critical forsuccessful fertilization, for example the spindles of the oocytes mightdisassemble within minutes during temperature change.

The wall structure utilized on commercially available well plates haslower wall base level than the level of the bottom, not allowing thecontact between bottom surface and heated working surface below.

Within IVF the rim elevating the bottom of generic dishes and plates area disadvantage since it introduces an air gap between the surface of theheated working table and the bottom of the dish or well. As air is not agood heat transporter, the temperature at the elevated bottom of thedishes and wells can be hard to control on heated working surfaces.Often the oocyte/embryo manipulations are done within a laminar air flowhood. The laminar air flow creates air movements which makes the controlof the temperature more difficult.

However removing the rim below the polymer specimen container such as ona commercially available Petri dish, reduces the structural integrity ofthe polymer container and increases the likelihood of distortion of thebottom surface during solidification of the polymer container duringproduction, as well as increasing the risk of scratching the surfaceduring stacking for packaging, transportation and incubation.

Another problem is related to traceability of specimens frompatients/donors in the various standard containers used during IVFprocedure, is the fact that traditional dishes are of a Petri dish typeor well plate type, where the container is fully covered by a lid.Identification can be made on the lid, however this lid can potentiallyfit other dishes of similar type potentially leading to mismatch ofspecimens and patients/donors.

It would be highly desirable to provide a specimen container allowingthe flat bottom to be in contact with the heated working table, whileallowing the stacking of the container without scratching the bottomsurface.

It would further be desirable that the container had structurecomprising area for identification means not being on the detachablelid.

OBJECT OF THE INVENTION

It is an object of this invention to provide a polymer specimencontainer having improved temperature transfer, i.e. an improved abilityof transferring heat from heated working surface. As temperaturetransfer is meant the ability of transferring heat, i.e. keeping thetemperature in the polymer specimen as close as possible to thetemperature of the heated working surface.

It is further an object of this invention to provide a lid structureallowing stacking of the containers with lid without scratching thebottom surface.

It is a further object of this invention to provide improved means foridentification of specimens.

It is a further object of the present invention to provide analternative to the prior art.

These and other objects are achieved by the aspect and embodiments ofthe invention described and shown herein.

SUMMARY OF THE INVENTION

Thus, the above described object and several other objects are intendedto be obtained in a first aspect of the invention by providing a polymerspecimen container comprising: a specimen carrier, said carriercomprising a bottom base externally flat connected to a wall, said wallcomprising an open hollow structure adapted to avoid twisting of saidbottom base during the polymer specimen container production process.

The main effect of said structure is to secure contact between flatbottom base and working table below.

In some embodiments said open wall structure may have its lowest levelabove the lowest level of the bottom base.

The invention is particularly, but not exclusively, advantageous toprovide a polymer specimen container having improved temperaturetransfer from heated working surface through the bottom of the containerto the specimens by securing full contact between the downwardly flatbottom of the container and the heated working surface, utilizing anopen wall construction with ridges and recesses for stabilizing againstdistortion of the flat bottom surface of the container as well asutilizing a wall base being above the level of the bottom base.

By providing adapted means for contacting the ridges and recesses of theopen wall construction, the invention has also the advantage of allowingstacking of the containers with lid without scratching the bottomsurface ensuring full visibility of the specimens through thetransparent bottom surface during use.

The invention is particularly, but not exclusively, advantageous toprovide improved means for identification of specimens by providing areafor identification on the open wall construction, which is not coveredby the lid.

This invention relates to an improved specimen container for thehandling, micro manipulation, micro injection and/or biopsy ofspermatozoa, sperm precursor cells, oocytes, pre-implantation embryos,zygotes, blastocysts, embryonic cells and stem cells, collectivelyreferred to hereinafter as “specimen” or “specimens”.

The specimens must be held as closely as possible at a stable 37 degreesCelsius, especially during critical procedures such as IVF, making useof heated working surface below the specimen container often placed witha laminar airflow hood. As air, and especially moving air, is not anoptimal heat conductor, close contact between the bottom of the specimencontainer and the heated working table is desirable.

The high degree of flatness of the bottom base is achieved during thesolidification of the polymer material of the polymer specimen containerduring its production.

The open hollow wall structure secures stability to the specimen carrierpreventing twisting or distortion of the bottom base, therebyfacilitating the formation of a bottom base with a high degree offlatness with no need for structural reinforcement structures below thebottom base.

Flat is herein defined as substantial parallel to the surface on whichthe container is placed, e.g. zero degree of inclination in respect tothe surface on which the container is placed.

In some embodiment the open hollow structure comprises recesses andridges adapted to provide stable support to specimen carrier, therebyavoiding twisting of said bottom base during said polymer specimencontainer production process.

In some embodiment the open wall structure has its lowest level abovethe lowest level of said bottom base externally flat. This allows for acompletely flat surface of the external bottom base.

In another embodiment the polymer specimen container further comprises alid, said lid comprising a top and a bottom surface, wherein said topsurface comprises protrusions complementary to at least one of therecesses in said open hollow wall structure, thereby avoiding scratchingof the bottom surface during stacking.

Complementary is herein defined as to be able to be combined so that theprotrusions in the top surfaces of the lid matches the recesses on theopen wall structure so that the protrusions can be contained into therecesses allowing for stacking of the polymer specimens containersavoiding risk of contact to the bottom base of the containers, andthereby avoiding scratching of the bottom surface.

In another embodiment the open hollow structure comprises identificationmeans.

The identification means may so be placed on, above or below the openhollow structure of the wall or in combinations hereof.

Examples of identification means may be for examples barcode,inscription, embossed text, writing or RFID-tags. The wall base levelabove level of the bottom base allows barcodes or other identificationmeans to be placed on the container and bend around the wall basewithout interfering with the temperature transfer to the flat bottombase remaining in contact with the working surface. Identification meansmay so be placed in different areas on, above or below the open hollowstructure of the wall or combinations hereof.

The bottom base externally flat is characterized by an internal surface,where the specimen is carried and contained and an external surface incontact with the external environment.

In some embodiments at least part of the internal surface of said bottombase is treated so as to modify its surface properties. For example saidinternal surface of said bottom base externally flat may befunctionalized by surface modification.

In another embodiment the invention has a circular or substantiallysquare outer geometry making microscopy easy using conventional rotationof the container or making use of more modern XY stage stations.

In another embodiment the invention has a circular innermost wallstructure situated in the center of the container structure ensuringcentrically rotations of the well if the dish is to be rotated formicroscopy purposes.

In another embodiment the bottom base externally flat comprises one ormore individually separated wells. These wells, when the polymerspecimen container is in use holds liquid medium sustaining thespecimens. The individually separated wells allows the wells to behomogeneously temperate as well as ensure no cross-contamination fromone well to another. If drop culture is used a potentially collapsingdrop will not interfere with other drops within the same container.

In another embodiment the internal surface of the bottom base of saidindividually separated wells has an inclination. This inclination, fromfew degrees up to 45°, allows specimens to settle not contacting thewall of the well. This in turn allows easy manipulation of the specimen.

This solves the problem of small specimens e.g. embryos hiding at thecorner between the wall and the bottom of the well. The slope allows thespecimen to gradually be transported by gravity towards the bottom whereit can easily be located by microscopy and easily accessed bymanipulating tools.

In another embodiment of the invention at least the bottom of the wellsis functionalized by surface modification.

The container can be used for a wide range of specimens of human andanimal cells, embryos, embryonic cells, oocytes, spermatozoa, spermprecursor cells, blastocysts and stem cells with all such specimensreceiving the same benefits

In a particular embodiment of the invention the intended use is forArtificial Reproductive Technology,

In a second aspect the invention provides a method for producing apolymer specimen container wherein the presence of an open hollowstructure comprising recesses and ridges prevents distortion of saidflat bottom base during said polymer specimen carrier solidification.

The first and other aspect of the present invention may each be combinedwith any of the other aspects and embodiments.

Other aspects, advantages and novel features of the invention willbecome more apparent from the following detailed description of theinvention when considered in conjunction with the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

The polymer specimen container according to the invention will now bedescribed in more detail with regard to the accompanying figures. Thefigures show one way of implementing the present invention and is not tobe construed as being limiting to other possible embodiments fallingwithin the scope of the attached claim set.

FIG. 1a shows a top view of an exemplary container and FIG. 1b is thecorresponding lid and FIG. 1c is the container with lid not covering theidentification area in accordance with embodiments of the invention.

FIG. 2a shows a top view and FIG. 2b shows a cross-sectional view of anexemplary container having a secondary apperance with an innermost walland an outer most wall and an area for identification here between inaccordance with embodiments of the invention.

FIG. 3 shows a side view of an exemplary container without lid having abottom surface being downwardly flat and having a wall base level higherthan the bottom base level in accordance with embodiments of thisinvention.

FIG. 4 shows a cross-sectional view of the lid having protrusions inaccordance with embodiments of the invention.

FIG. 5 shows a back-side view of to exemplary containers with lidstacked on top of each other. The lid having protrusions in accordancewith embodiments of the invention making contact with reassesses on theopen wall structure allowing stacking of containers or carriers withoutscratching the bottom surface.

FIG. 6 shows a top view of an exemplary container having a thirdappearance with separated wells in accordance with embodiments of theinvention.

FIG. 7 shows a top view of an exemplary container having a forthappearance with a slope in each separated well in accordance withembodiments of the invention.

FIG. 8 shows a top view on an exemplary container having a fifthappearance with a centred well in accordance with embodiments of theinvention.

FIG. 9a shows a top view and FIG. 9b the corresponding lid and FIG. 9cthe cross-sectional view of an exemplary container having a sixthappearance in accordance with some aspect of the invention.

FIG. 10 shows a top view of an exemplary container in accordance withembodiments of this invention alongside two commercially availablecontainers, all placed on an external surface heated to 37 degreesCelsius, illustrating temperature transfer and homogeneity in thedifferent containers.

FIG. 11 shows a top view of a commercially available Petri dishalongside an exemplary container in accordance with embodiments of thisinvention, both placed on an external surface heated to 37 degreesCelsius, illustrating temperature transfer and homogeneity in thedifferent containers.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

With reference to the drawings for purpose of illustration, the presentinvention is embodied in a dish (FIG. 1a ) with a separate lid (FIG. 1b) not covering the dedicated identification area (FIG. 1c ) securingconstant visibility during use.

On FIG. 2 the lid fits into structures (4,9). A writing area (3) ispositioned between the innermost wall (5) and the outermost wall (2)being part of the open wall structure on one side of the bottom surface,just like the innermost wall (8) and the outermost wall (10) also beingpart of the open wall structure. The open wall structure hasreinforcements by ridges and recesses (11,12,15,16). The reinforced openwall structure prevents distortion of the polymer container duringpolymer solidification. The open wall structure is interconnected to abottom base (13) having a surface being downwardly flat. The bottom base(13) has a base level (18) lower than the lowest level (1) of any otherstructures on the article securing full contact of the bottom surface tothe underlying often heated working surface and thereby securing optimaltemperature transfer to the specimens to be contained inside thecontainer e.g. in a well (14). The well could be separated by a wallstructure having a slope (6,7) between the actual wall structure and thebottom structure, securing positioning of the specimen away from thewall structure of the well. If a label, barcode or RFID tag is to beplaced below the container for imaging purpose it may be placed on theoutermost wall structure (2) and bend underneath the slightly elevatedwall structure and be supported by a protrusion (17).

The lid in FIG. 4 has a rim (1) to secure stability when positioned onthe container. The lid also has a protrusion (2) which makes contact onridges and recesses on the open wall structure of an adjacent containerduring stacking, making sure the bottom of the container is notscratched during stacking as well as to facilitate easy grapping.Stacking is illustrated on FIG. 5.

The lid, FIG. 1b , may further have small protrusions (1) securing gasexchange between the outside of the container and the inside of thecontainer during use.

Definitions

Sterile means free from living bacteria and other microorganisms.Sterilisation is the process by which living organisms are removed orkilled to the extent that they are no longer detectable in standard cellculture media in which they had previously proliferated. SterilityAssurance Level of six-log reduction (also termed log of minus 6reduction or ten to the minus six reductions) is considered acceptablefor many medical devices.

Open wall structure means a wall structure consisting of a non-closedwall structure persisting in more than one plane.

Innermost wall structure means the wall nearest to the inside of thecontainer or carrier.

Outermost wall structure means the wall nearest to the outside of thecontainer or carrier.

Downwardly flat bottom base and externally flat bottom base means thatthe bottom area facing downwards, towards the outside of the container,is flat. The part of the bottom area facing upwards, towards the insideof the container could be flat, but could also contain well structures.

Square with or without rounded corners means a substantially squaregeometry with corner angle of 90 degrees or rounded.

Center of the dish means the center of the dish confined by theoutermost wall.

Individually separated wells means wells separated by a wall structureor embedded in the bottom structure in a way so liquid in one well isseparated from liquid in another well.

Slope means a ramp with an inclination between 0 and 90 degrees. A slopein the bottom of a well may interconnect the bottom area with the wallstructure of the well.

Functionalized by surface modification means modifying the surfaceproperties e.g. by use of electric discharge, plasma, biologicalmolecules, irradiation, structuring, material embedding, chemical ormolecular vapor deposition or other means.

Identification marking means marking with text, print, labels,inscription, transmitter or other means in order trace container andcontent

RFID means Radio-frequency Identification. RFID is a technology thatincorporates the use of electromagnetic spectrum to uniquely identify anobject.

Assisted Reproductive Technology (ART) is a general term referring tomethods used to achieve Pregnancy by artificial or partially artificialmeans. It is a reproductive technology used primarily in Infertilitytreatments treatments, such as InVitro Fertilisation.

In Vitro Fertilization (IVF) is a laboratory procedure in which spermare placed with an unfertilized egg in a dish to achieve fertilization.

XY-stage station means automated or manual positioning table deliveringsmooth and accurate two-dimensional motion.

Collapsing drops means liquid drops placed on a solid support, e.g.bottom surface of a polymer container, and not having a measurablecontact angle with the solid support, a phenomenon occurring when liquidis placed on hydrophilic surfaces and a well defined drop withmeasurable contact angle cannot be maintained.

Protrusion means something that bulges out or is protuberant and therebyraises above the surrounding support.

Meiose activation means activating cell division necessary for sexualreproduction

Chemical vapor deposition (CVD) means a chemical process used to producehigh-purity, high-performance solid materials. The process is generallyused in the semiconductor industry to produce thin films. In a typicalCVD process, the substrate is exposed to one or more volatile precursorswhich react and precipitate or decompose on the substrate surface toproduce the desired deposit

Molecular vapor deposition (MVD) means an enhancement of theconventional vapor deposition of ultra-thin layers. It is a non-plasmavacuum process where volatile components in gas phase react to form asolid deposit and gaseous by-products. In MVD the reagentsconcentrations control the deposition rate. Reagents are distributeduniformly in the system by means of pressure and MVD is performed as abatch process so that no new reagent is added to the process during thedeposition. MVD is not an invasive technique, the low temperature, theabsence of plasma and the introduction of reagents as gases do notdamage the substrate.

Base height means the height of the supporting base of any structure onthe article.

Bottom base means the lowest base of the bottom surface.

Wall base means the lowest surface of the open wall structure. When thewall is at one end connected to the bottom surface and stretching onlyonto one side of the bottom surface, the wall base is defined as beingabove the level of the bottom base.

Flat is herein defined as substantial parallel to the surface on whichthe container is placed, e.g. zero degree of inclination in respect tothe surface on which the container is placed.

Working surface is defined as the surface onto which the container orcarrier in placed during use. E.g. this working surface may be a heatedor non-heated surface, a structured surface within an incubator, asurface within a laminar airflow hood, the surface of the lid of anothercontainer or carrier and more.

EXAMPLES

Polymer specimen container with open wall structure, flat bottom base incontact with working surface, area for identification, and optionallyslope between wall structure of the well and bottom of the well

The polymer container of the present invention may have any suitablesize for the intended use.

In particular polymer container being substantially square or squarewith rounded corners having a side length of 25 mm or less, such as 20mm or less, 15 mm or less, 10 mm or less such as approximately 7.5 mm. Asubstantially square geometry is useful during microscopy using an XYstage moving the container in X- and Y directions.

In particular polymer container being substantially circular having adiameter of 120 mm or less, such as 90 mm or less, such as 60 mm orless, such as 50 mm or less, such as 40 mm or less. A substantiallycircular geometry is useful during microscopy rotating the container byhand.

In particular polymer container being substantially square or squarewith rounded corners containing circular well(s) having any suitablesize for the intended use, in particular well diameter of 90 mm or less,60 mm or less, 45 mm or less, 35 mm or less, 10 mm or less, 5 mm orless. A substantially square or square dish with rounded cornerscontaining a circular well with center at the center of the container isuseful during microscopy rotating the container by hand. Centered or anon-centered well is useful for micro drop culture as well as collectionand transferring specimens. Several wells within the same container areuseful for culturing embryos separately and securing no crosscontamination between embryo cultures, also in the event of collapsingdrops.

The well(s) may have any shape suitable for intended use. When usedduring procedures where tools are applied from left and right hand sideat the specimens within the well, a circular, oval or rectangular wellshape may be preferred. When used during embryo culture a circular wellmay be preferred. The individual wells may optionally have a slopebetween the flat bottom of the well and the well structure, useful forpreventing small specimens to hide in the otherwise sharp angles. Theslope ensures that the small specimens such as embryos are transferredto the bottom of the well by gravity and easily accessible at a knowndistance from the wall of the well. The slope of the present inventionmay have suitable size for intended use, in particular slope angle of 89degrees or less, 45 degrees or less, 30 degrees or less, such as 25degrees.

Some procedures require larger amounts of media within the container,some requires smaller amount of media, and some requires easy accesswith tools approaching the containers from the sides. The height of thecontainer may have any shape suitable for intended use, in particular 20mm or less, 15 mm or less, 10 mm or less, 9 mm or less, 8 mm or less, 7mm or less, 6 mm or less, 5 mm or less.

In contrast to a traditional Petri dish the invention provides an openwall structure

One benefit being that part of the area on the open wall structureprovides an identification area suitable for marking, engraving,labeling, barcode, RFID tag or the like. The Identification area of thepresent invention may have suitable size for the intended use. Inparticular area of 100 cm² or less, 50 cm² or less, 25 cm² or less, 10cm² or less, 8 cm² or less.

Another benefit being that the open wall structure interconnected by abottom structure being downwardly flat and said bottom base having alower base level than the base of the wall structure is that the bottomsurface is being in close contact with the external working surfacebelow, which may be a heated table. The open wall structure adapted withrecesses and ridges prevents the injection molded flat bottom structureto twist during solidification of the heated polymer. The bottomstructure may have suitable size for the intended use. In particularthickness of 12 mm or less, 10 mm or less, 8 mm or less, 6 mm or less, 4mm or less. The bottom structure may also embed well structures. Thetemperature transfer from the external heated surface to the specimensis thus optimized for the individual container, as well as ease ofprocedure is optimized when several various containers using sameprinciple are used during an IVF procedure.

A third benefit being that the lid may be fitted to cover the areaconstraint by the innermost wall structure, while allowing theidentification area to not be covered by the lid for easyidentification.

The lid has protrusions on the upper side contacting recesses and ridgeson the open wall structure during stacking of the containers on top ofeach other. This reduces risk of scratching the bottom, which can leadto reduced visibility of the specimens. This feature also ensures thatonly a container with its lid is gripped when reaching out towards thestacked products.

The lid having protrusions on the lower side ensuring that gas exchangecan take place between the content of the container and the outside ofthe container with lid, e.g. during placement in gas controlledincubator.

The polymer container of the present invention is formed from a heatedpolymer, in particular a thermoplastic compound. Non-limiting examplesof thermoplastic compounds that may be used are acrylonitrile butadienestyrene (ABS), acrylic, celluloid, cellulose acetate, Ethylene-VinylAcetate (EVA), Ethylene vinyl alcohol (EVAL), Flouroplastics, LiquidCrystal polymer (LCP), polyacetal, polyacrylate, polyacrylonitrile,polyamide, polyamide-omide (PAI polychlorotrifluoroethylene (PCTFE),polyethylene terephthalate (PET), polycyclohexylene dimethyleneterephthalate (PCT), polycarbonate (PC), polyhydroxyalkanoates (PHAs),polyketone (PK), polyester, polyethylene (PE), polyetherketone (PEEK),polyetherimide (PEI), polyesthersulfone (PES), polyethylenechlorinates(PEC), polyimide (PI), polylactic acid (PLA), polymethylpentene (PMP),polyphenylene oxide (PPO), polyphenylene sulfide (PPS), polyphthalamide(PPA), polypropulene (PP), polystyrene (PS), polysulfone (PSU),polyurethane (PU), polyvinyl acetate (PVA), polyvinyl chloride (PVC),polyvinylidenechloride (PVDC) and styrene-acrylonitrile (SAM), or mixesor copolymers thereof.

In a particularly preferred embodiment the polymer container is formedfrom a polymeric material which is biocompatible.

The polymeric material may be selected such that it has a primarilyhydrophobic or primarily hydrophilic surface or primarily in-between. Ina particular embodiment the surface of the polymer article may bemodified to make it in-between, e.g. by coating or by use of corona orplasma treatment. The polymers are generally hydrophobic by nature andthe strength and time of the coating process determines the degree ofhydrophilicity obtained.

Process for Producing a Polymer Container Optionally Functionalized bySurface Modification

Typical methods used in the mass production of polymer articles areinjection molding. Injection molding is performed by heating a suitablepolymer until molten, injecting the molten polymer into a mold, allowingthe polymer to cool and harden, and removing the molded article from themold after solidification.

The surface of the polymer container is optionally made more hydrophilicto facilitate the formation of drops of media for culturing embryos, aswell as made sterile to prevent contamination. Typically the surface ofthe polymer container is modified by corona or plasma treatment toincrease surface wettability through electric discharge.

Sterilisation by irradiation (beta or gamma), steam autoclave, ethyleneoxide, chemical disinfectants or dry heat are the typical sterilizationmethods used.

The sterile polymer container may further be functionalized bystructuring the surface during the actual injection molding step and/orsimply by wet chemical coating or vapor coating following the injectionmolding.

A functionalisation by adding structure to the surface of the polymercontainer during molding may be in the form of molded nano or microstructures within the polymer or by integrating a porous scaffold intothe surface of the polymer container during injection molding. Thestructures allows specimens in contact with the structures within thecontainer to obtain more 3D like morphology as well as intercommunicatewith other specimens through the medium travelling unhindered throughthe structures retaining the specimens.

Coating using wet chemical process covalently bonds biological moleculesto the polymer surface to facilitate specific functionality in relationto interaction with the surroundings being it meiose activation, cellattachment, molecule capture or separation and/or purificationprocesses.

Coating using vapor process deposits oxide layers onto the surface ofthe polymer container. Examples of oxide layers may be Al₂O₃, SiO₂,TiO₂, ZrO₂, CeO₂ or a mixture or a multilayer structure thereof. Oxidelayers may be deposited by Chemical Vapor deposition (CVD) or preferablyby Molecular vapor deposition (MVD).

Experimental Example

The following examples serve to more fully describe the functionality ofthe externally flat bottom in contact with external heated surfacecompared to traditional containers for IVF. These examples do not limitthe scope of the disclosure, but rather are presented for illustrativepurposes.

Example 1 Temperature Transfer to Polymer Container

Two conventionally polymer containers with wells and a polymer containeraccording to this invention are placed on a metal plate heated to 37.2Degrees Celsius.

In FIG. 10 the two conventional polymer containers (B and C) have abottom situated approx 0.5 mm above the heated plate surface. Thecontainer (A) according to this invention has full contact between thebottom of the container and the heated plate.

Each well is filled with liquid to obtain equal liquid height.

Allow 5 minutes for the heated plate to transfer heat to the containers.

Use a thermo sensitive camera to take an image of the heat distributionwithin the containers.

FIG. 10 illustrates the more optimal heat transfer from the heated plateto the liquid within the 5 well container (A) according to this exampleand this invention compared to commercially available 5 well (B) and 4well (C) plates.

One conventionally polymer Petri dish and a polymer container accordingto this invention are placed on a metal plate heated to 37.2 DegreesCelsius.

In FIG. 11 the one conventional Petri dish (D) has a bottom situatedapprox 0.5 mm above the heated plate surface. The container (E)according to this invention has full contact between the bottom of thecontainer and the heated plate.

Each container is filled with liquid to obtain equal liquid height.

Allow 5 minutes for the heated plate to transfer heat to the containers.

Use a thermo sensitive camera to take an image of the heat distributionwithin the containers.

FIG. 11 illustrates the more optimal heat transfer from the heated plateto the liquid within the container (E) according to this example andthis invention compared to commercially available Petri dish (D).

Clearly, the polymer specimen of the invention has an improved abilityof transferring heat to the liquid therein contained. In particular, thepolymer specimen of the invention transports heat better thancommercially available containers. The polymer specimen structurecomprising ridges and recesses allows for a better contact between theheated plate and the bottom of the polymer specimen.

Although the present invention has been described in connection with thespecified embodiments, it should not be construed as being in any waylimited to the presented examples. The scope of the present invention isset out by the accompanying claim set. In the context of the claims, theterms “comprising” or “comprises” do not exclude other possible elementsor steps. Also, the mentioning of references such as “a” or “an” etc.should not be construed as excluding a plurality. The use of referencesigns in the claims with respect to elements indicated in the figuresshall also not be construed as limiting the scope of the invention.Furthermore, individual features mentioned in different claims, maypossibly be advantageously combined, and the mentioning of thesefeatures in different claims does not exclude that a combination offeatures is not possible and advantageous.

The following numbered items provide in term of conceptual statementsfurther disclosure of the present subject matter.

1. A polymer specimen container comprising:

a specimen carrier, said carrier comprising a bottom base externallyflat connected to a wall, said wall comprising an open hollow structureadapted to avoid twisting of said bottom base during the polymerspecimen container production process.

2. A polymer specimen carrier according to item 1, wherein said openhollow structure comprises recesses and ridges adapted to provide stablesupport to said specimen carrier.

3. A polymer specimen carrier according to any of the preceding itemswherein said open wall structure has its lowest level above the lowestlevel of said bottom base externally flat.

4. A polymer specimen container according to any of the preceding itemsfurther comprising a lid, said lid comprising a top and a bottomsurface, wherein said top surface comprises protrusions complementary toat least one of said recesses or ridges in said open hollow wallstructure.

5. A polymer specimen carrier according to any of the preceding itemswherein said open hollow structure comprises area for identificationmeans.

6. A polymer specimen carrier according to any of the preceding items,wherein said identification means is in the form of barcode,inscription, embossed text, writing or RFID tag.

7. A polymer specimen carrier according to any of the preceding items,wherein at least part of the internal surface of said bottom baseexternally flat is treated so as to modify its surface properties.

8. A polymer specimen carrier according to any of the preceding items,wherein said open wall structure forms substantially a square with orwithout rounded corners or being substantially circular.

9. A polymer specimen carrier according to any of the preceding items,wherein said open wall structure having an innermost wall structureforming a well substantially square with or without rounded corners, orbeing substantially circular.

10. A polymer specimen carrier according to any of the preceding items,wherein said innermost wall structure forms a circular well, wherein thecentre of said circular well is substantially identical to the centre ofthe said specimen carrier.

11. A polymer specimen carrier according to any of the preceding items,wherein said flat bottom base externally flat comprises one or moreindividually separated wells for holding liquid medium sustaining thespecimens during procedure.

12. A polymer specimen carrier according to item 11, wherein theinternal surface of the bottom base of said individually separated wellshas an inclination.

13. A polymer specimen carrier according to any of the preceding itemscomprising at least an innermost and an outermost wall structure, saidinnermost wall structure interconnected by a sterile bottom base beingexternally flat and having equal or lower base height than any othercarrier structures, optimizing bottom base contact to a heated workingsurface and thereby improving heat transfer to the inside of thecarrier.

14. A polymer specimen carrier according to any of the preceding itemswherein the specimens are oocytes, embryos, blastocysts, spermatozoa orstem cells.

15. The use of a polymer specimen container according to any of thepreceding items within the area of Assisted Reproductive Technology.

16. A method for producing a polymer specimen container according toitems 2-15 wherein the presence of an open hollow structure comprisingrecesses and ridges prevents distortion of said flat bottom base duringsaid polymer specimen carrier solidification.

1. (canceled)
 2. A specimen container comprising: a well configured toreceive a specimen comprising a liquid, the well comprising a bottomwall with an externally flat bottom surface, and an open wall structuresurrounding the well, the open wall structure comprising: an inner walland an outer wall, an upper wall connecting a top portion of the innerwall to a top portion of the outer wall, and at least one ridgeextending downwardly from the upper wall and positioned between theinner wall and the outer wall, wherein the externally flat bottomsurface of the bottom wall of the well is at a lower position than anyportion of the open wall structure such that the externally flat bottomsurface is configured to rest directly on and fully contact a heatedplate when the specimen container is placed thereon; and a lidconfigured to cover at least the well of the specimen container, the lidcomprising: a rim extending downwardly from a bottom surface of the lid,the rim configured to be at least partially received within a recess onthe upper wall of the open wall structure when the lid is placedthereon, and one or more protrusions extending upwardly from a topsurface of the lid, the one or more protrusions configured to contactand support an additional specimen container when stacked thereonwithout contacting an externally flat bottom surface of the additionalspecimen container.
 3. The specimen container of claim 2, wherein theopen wall structure further comprises an identification area portionpositioned on the upper wall of the open wall structure, wherein theidentification area portion is not covered by the lid when the lid isplaced thereon.
 4. The specimen container of claim 3, wherein theidentification area portion comprises a barcode, an inscription, anembossed text, a writing or an RFID tag.
 5. The specimen container ofclaim 2, wherein an upper surface of the bottom wall of the wellcomprises one or more sloped surfaces configured such that the specimenis directed toward a bottom of the well.
 6. The specimen container ofclaim 5, wherein the one or more sloped surfaces comprise an angle ofabout 45 degrees or less measured with respect to the externally flatbottom surface.
 7. The specimen container of claim 2, wherein thespecimen container comprises a plurality of wells surrounded by the openwall structure, each of the plurality of wells separated by walls andcomprising bottom walls with externally flat bottom surfaces.
 8. Thespecimen container of claim 2, wherein the well comprises a circular oroval shape.
 9. The specimen container of claim 8, wherein the specimencontainer comprises a square or rectangular shape.
 10. The specimencontainer of claim 8, wherein the specimen container is formed from apolymer material.
 11. The specimen container of claim 10, wherein theopen wall structure is configured to prevent twisting of the bottom wallof the well during solidification of the polymer during manufacturing,thereby maintaining the externally flat bottom surface.
 12. The specimencontainer of claim 2, wherein the specimen comprises spermatozoa, spermprecursor cells, oocytes, pre-implantation embryos, zygotes,blastocysts, embryonic cells or stem cells.
 13. The specimen containerof claim 12, wherein the specimen container is configured for an invitro fertilization (IVF) procedure, wherein the specimen container ispositioned on the heated plate such that the externally flat bottomsurfaces fully contact the heated plate and the specimen is held atapproximately 37 degrees Celsius.